recombinant mouse il10 Search Results


mouse recombinant il 10 ril 10  (R&D Systems)
95
R&D Systems mouse recombinant il 10 ril 10
Mouse Recombinant Il 10 Ril 10, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1 recombinant mouse il 10 receptor alpha  (R&D Systems)
92
R&D Systems 1 recombinant mouse il 10 receptor alpha
1 Recombinant Mouse Il 10 Receptor Alpha, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant mouse il 1 α  (R&D Systems)
91
R&D Systems recombinant mouse il 1 α
Recombinant Mouse Il 1 α, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant murine il10 r d systems  (R&D Systems)
95
R&D Systems recombinant murine il10 r d systems
Figure 3. GCN2 levels regulate macrophage polarization during skin wound healing (A) Flow cytometry analysis of F4/80+CD11b+ skin wound macrophages (gated on CD45+ cells) from WT and GCN2KO mice at 3 or 5 dpi (n = 3). (B) Flow cytometry analysis of CD86 expression in skin wound macrophages from WT and GCN2KO mice at 3 or 5 dpi (n = 3). (C) Flow cytometry analysis of CD206 expression in skin wound macrophages from WT and GCN2KO mice at 3 or 5 dpi (n = 3). (D) CD206, <t>Il10,</t> Tgfb1, and Vegfa expression in 3 or 5 dpi skin macrophages from WT and GCN2KO mice analyzed by real-time PCR (n = 3). (E) Cx3cr1 and Il6 expression in 3 or 5 dpi skin macrophages from WT and GCN2KO mice analyzed by real-time PCR (n = 3). (F and G) Rose diagrams showing all highly expressed GCN2KO genes and all highly expressed WT genes at 3 (F) and 5 dpi (G). (H–J) Heatmap showing differentially expressed M1 (H), M2a (I), and M2c (J) marker genes in WT and GCN2KO mice. Macrophages were obtained from sorted skin wound macrophages (A–G) or peritoneal macrophages (H–J). Data represent three independent experiments and are presented as mean ± SD. Each independent experiment consists of at least three technical replicates. *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA. See also Figure S3.
Recombinant Murine Il10 R D Systems, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+il10/Recombinant+Mouse+IL-10+Protein/pm38175755-206-288-291
Average 95 stars, based on 1 article reviews
recombinant murine il10 r d systems - by Bioz Stars, 2026-09
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il 10 r d system  (R&D Systems)
92
R&D Systems il 10 r d system
Figure 3. GCN2 levels regulate macrophage polarization during skin wound healing (A) Flow cytometry analysis of F4/80+CD11b+ skin wound macrophages (gated on CD45+ cells) from WT and GCN2KO mice at 3 or 5 dpi (n = 3). (B) Flow cytometry analysis of CD86 expression in skin wound macrophages from WT and GCN2KO mice at 3 or 5 dpi (n = 3). (C) Flow cytometry analysis of CD206 expression in skin wound macrophages from WT and GCN2KO mice at 3 or 5 dpi (n = 3). (D) CD206, <t>Il10,</t> Tgfb1, and Vegfa expression in 3 or 5 dpi skin macrophages from WT and GCN2KO mice analyzed by real-time PCR (n = 3). (E) Cx3cr1 and Il6 expression in 3 or 5 dpi skin macrophages from WT and GCN2KO mice analyzed by real-time PCR (n = 3). (F and G) Rose diagrams showing all highly expressed GCN2KO genes and all highly expressed WT genes at 3 (F) and 5 dpi (G). (H–J) Heatmap showing differentially expressed M1 (H), M2a (I), and M2c (J) marker genes in WT and GCN2KO mice. Macrophages were obtained from sorted skin wound macrophages (A–G) or peritoneal macrophages (H–J). Data represent three independent experiments and are presented as mean ± SD. Each independent experiment consists of at least three technical replicates. *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA. See also Figure S3.
Il 10 R D System, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+il10/Recombinant+Mouse+IL-10+Protein%2C+CF/pm36099917-181-0-1
Average 92 stars, based on 1 article reviews
il 10 r d system - by Bioz Stars, 2026-09
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recombinant mouse eotaxin  (R&D Systems)
90
R&D Systems recombinant mouse eotaxin
FIGURE 3. bmEos exhibit chemotaxis toward <t>recombinant</t> mouse <t>eotaxin-1.</t> bmEos from day 10 cultures were used to measure chemotaxis in the presence of a mouse eotaxin-1 gradient (f), determined as percent- age of cells migrating over control (no eotaxin); chemokinesis (migration measured in response to eotaxin-1 in both chambers) is indicated by . Indistinguishable results were obtained using bmEos from day 12 cultures. Mean SEM of three separate cultures.
Recombinant Mouse Eotaxin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+il10/Recombinant+Mouse+IL-10+Protein%2C+CF/pm18768855-80-0-3
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recombinant mouse eotaxin - by Bioz Stars, 2026-09
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mouse interleukin 10 il 10  (Beijing Solarbio Science)
93
Beijing Solarbio Science mouse interleukin 10 il 10
FIGURE 3. bmEos exhibit chemotaxis toward <t>recombinant</t> mouse <t>eotaxin-1.</t> bmEos from day 10 cultures were used to measure chemotaxis in the presence of a mouse eotaxin-1 gradient (f), determined as percent- age of cells migrating over control (no eotaxin); chemokinesis (migration measured in response to eotaxin-1 in both chambers) is indicated by . Indistinguishable results were obtained using bmEos from day 12 cultures. Mean SEM of three separate cultures.
Mouse Interleukin 10 Il 10, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+il10/Recombinant+Mouse+IL-10%2FInterleukin-10/pmc12139202-59-0-15
Average 93 stars, based on 1 article reviews
mouse interleukin 10 il 10 - by Bioz Stars, 2026-09
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il 10  (Boster Bio)
90
Boster Bio il 10
FIGURE 3. bmEos exhibit chemotaxis toward <t>recombinant</t> mouse <t>eotaxin-1.</t> bmEos from day 10 cultures were used to measure chemotaxis in the presence of a mouse eotaxin-1 gradient (f), determined as percent- age of cells migrating over control (no eotaxin); chemokinesis (migration measured in response to eotaxin-1 in both chambers) is indicated by . Indistinguishable results were obtained using bmEos from day 12 cultures. Mean SEM of three separate cultures.
Il 10, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+il10/IL-10+Interleukin-10+Mouse+Recombinant+Protein/pmc07403330-378-5-30
Average 90 stars, based on 1 article reviews
il 10 - by Bioz Stars, 2026-09
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recombinant mouse il-10 (cys167tyr) protein  (Bio-Techne corporation)
91
Bio-Techne corporation recombinant mouse il-10 (cys167tyr) protein
FIGURE 3. bmEos exhibit chemotaxis toward <t>recombinant</t> mouse <t>eotaxin-1.</t> bmEos from day 10 cultures were used to measure chemotaxis in the presence of a mouse eotaxin-1 gradient (f), determined as percent- age of cells migrating over control (no eotaxin); chemokinesis (migration measured in response to eotaxin-1 in both chambers) is indicated by . Indistinguishable results were obtained using bmEos from day 12 cultures. Mean SEM of three separate cultures.
Recombinant Mouse Il 10 (Cys167tyr) Protein, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
recombinant mouse il-10 (cys167tyr) protein - by Bioz Stars, 2026-09
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recombinant mouse il-10 (cys167tyr) protein, cf  (Bio-Techne corporation)
90
Bio-Techne corporation recombinant mouse il-10 (cys167tyr) protein, cf
FIGURE 3. bmEos exhibit chemotaxis toward <t>recombinant</t> mouse <t>eotaxin-1.</t> bmEos from day 10 cultures were used to measure chemotaxis in the presence of a mouse eotaxin-1 gradient (f), determined as percent- age of cells migrating over control (no eotaxin); chemokinesis (migration measured in response to eotaxin-1 in both chambers) is indicated by . Indistinguishable results were obtained using bmEos from day 12 cultures. Mean SEM of three separate cultures.
Recombinant Mouse Il 10 (Cys167tyr) Protein, Cf, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+il10/Recombinant+Mouse+IL-10+(Cys167Tyr)+Protein%2C+CF/bio-techne+corporation___1023-ml-cf
Average 90 stars, based on 1 article reviews
recombinant mouse il-10 (cys167tyr) protein, cf - by Bioz Stars, 2026-09
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recombinant mouse adm protein  (Novus Biologicals)
90
Novus Biologicals recombinant mouse adm protein
FIGURE 3. bmEos exhibit chemotaxis toward <t>recombinant</t> mouse <t>eotaxin-1.</t> bmEos from day 10 cultures were used to measure chemotaxis in the presence of a mouse eotaxin-1 gradient (f), determined as percent- age of cells migrating over control (no eotaxin); chemokinesis (migration measured in response to eotaxin-1 in both chambers) is indicated by . Indistinguishable results were obtained using bmEos from day 12 cultures. Mean SEM of three separate cultures.
Recombinant Mouse Adm Protein, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+mouse+il10/Recombinant+Mouse+IL-10+Protein/pm22960424-114-8-19
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recombinant mouse adm protein - by Bioz Stars, 2026-09
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v08b16 recombinant mouse il 10 novoprotein  (Novoprotein)
86
Novoprotein v08b16 recombinant mouse il 10 novoprotein
FIGURE 3. bmEos exhibit chemotaxis toward <t>recombinant</t> mouse <t>eotaxin-1.</t> bmEos from day 10 cultures were used to measure chemotaxis in the presence of a mouse eotaxin-1 gradient (f), determined as percent- age of cells migrating over control (no eotaxin); chemokinesis (migration measured in response to eotaxin-1 in both chambers) is indicated by . Indistinguishable results were obtained using bmEos from day 12 cultures. Mean SEM of three separate cultures.
V08b16 Recombinant Mouse Il 10 Novoprotein, supplied by Novoprotein, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 3. GCN2 levels regulate macrophage polarization during skin wound healing (A) Flow cytometry analysis of F4/80+CD11b+ skin wound macrophages (gated on CD45+ cells) from WT and GCN2KO mice at 3 or 5 dpi (n = 3). (B) Flow cytometry analysis of CD86 expression in skin wound macrophages from WT and GCN2KO mice at 3 or 5 dpi (n = 3). (C) Flow cytometry analysis of CD206 expression in skin wound macrophages from WT and GCN2KO mice at 3 or 5 dpi (n = 3). (D) CD206, Il10, Tgfb1, and Vegfa expression in 3 or 5 dpi skin macrophages from WT and GCN2KO mice analyzed by real-time PCR (n = 3). (E) Cx3cr1 and Il6 expression in 3 or 5 dpi skin macrophages from WT and GCN2KO mice analyzed by real-time PCR (n = 3). (F and G) Rose diagrams showing all highly expressed GCN2KO genes and all highly expressed WT genes at 3 (F) and 5 dpi (G). (H–J) Heatmap showing differentially expressed M1 (H), M2a (I), and M2c (J) marker genes in WT and GCN2KO mice. Macrophages were obtained from sorted skin wound macrophages (A–G) or peritoneal macrophages (H–J). Data represent three independent experiments and are presented as mean ± SD. Each independent experiment consists of at least three technical replicates. *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA. See also Figure S3.

Journal: Cell reports

Article Title: Downregulation of nutrition sensor GCN2 in macrophages contributes to poor wound healing in diabetes.

doi: 10.1016/j.celrep.2023.113658

Figure Lengend Snippet: Figure 3. GCN2 levels regulate macrophage polarization during skin wound healing (A) Flow cytometry analysis of F4/80+CD11b+ skin wound macrophages (gated on CD45+ cells) from WT and GCN2KO mice at 3 or 5 dpi (n = 3). (B) Flow cytometry analysis of CD86 expression in skin wound macrophages from WT and GCN2KO mice at 3 or 5 dpi (n = 3). (C) Flow cytometry analysis of CD206 expression in skin wound macrophages from WT and GCN2KO mice at 3 or 5 dpi (n = 3). (D) CD206, Il10, Tgfb1, and Vegfa expression in 3 or 5 dpi skin macrophages from WT and GCN2KO mice analyzed by real-time PCR (n = 3). (E) Cx3cr1 and Il6 expression in 3 or 5 dpi skin macrophages from WT and GCN2KO mice analyzed by real-time PCR (n = 3). (F and G) Rose diagrams showing all highly expressed GCN2KO genes and all highly expressed WT genes at 3 (F) and 5 dpi (G). (H–J) Heatmap showing differentially expressed M1 (H), M2a (I), and M2c (J) marker genes in WT and GCN2KO mice. Macrophages were obtained from sorted skin wound macrophages (A–G) or peritoneal macrophages (H–J). Data represent three independent experiments and are presented as mean ± SD. Each independent experiment consists of at least three technical replicates. *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA. See also Figure S3.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC anti-mouse CD206 BioLegend Cat# 141703; RRID: AB_10900988 PE anti-mouse F4/80 BioLegend Cat# 123110; RRID: AB_893486 APC anti-mouse F4/80 Thermo Fisher Cat# 17-4801-82; RRID: AB_2784648 PE/Cy5 anti-mouse CD11b Thermo Fisher Cat# 15-0112-83; RRID: AB_468714 Bv510 anti-mouse CD11b BioLegend Cat# 101263; RRID: AB_2629529 PE anti-mouse CD124 BioLegend Cat# 144804; RRID: AB_2561730 FITC anti-mouse F4/80 Thermo Fisher Cat# 11-4801-82; RRID: AB_2637191 PE anti-mouse TNFa Thermo Fisher Cat# 12-7321-82; RRID: AB_466199 FITC anti-mouse CD86 eBioscience Cat# 105006; RRID: AB_313149 PE/Cy5 anti-mouse CD45 Thermo Fisher Cat# 15-0459-42; RRID: AB_10717815 APC anti-mouse CD45 BioLegend Cat# 103112; RRID: AB_312977 PE anti-mouse Ly6G BioLegend Cat# 127607; RRID: AB_1186104 PE anti-mouse CD115 BioLegend Cat# 135506; RRID: AB_1937253 PE/Cy7 anti-mouse CD45.1 BioLegend Cat# 110730; RRID: AB_1134168 Fixable Viability Dye eFluorTM 780 Thermo Fisher Cat# 65-0865-14; RRID: —— PERCP/CY5.5 anti-mouse CD45.2 BioLegend Cat# 109827; RRID: AB_893352 Brilliant Violet 510 anti-mouse CD11b BioLegend Cat# 101245; RRID: AB_2561390 Brilliant Violet 605 anti-mouse Ly6C BioLegend Cat# 128036; RRID: AB_2562353 b-actin Sigma Cat# A5441; RRID: AB_476744 Arginase1 Santa Cruz Cat# sc-20150; RRID: AB_2058955 GCN2 Cell Signaling Technology Cat# 3302s; RRID: AB_10694800 p-IkBa Cell Signaling Technology Cat# 92456s; RRID: AB_2267145 IkBa Abcam Cat# ab32518; RRID: AB_2801653 p-IKKa/b Cell Signaling Technology Cat# 14938; RRID: AB_2798653 IKKa Cell Signaling Technology Cat# 61294s; RRID: AB_2799606 P65 Cell Signaling Technology Cat# 6596s; RRID: AB_2128043 p-P65 Cell Signaling Technology Cat# 3033T; RRID: AB_331284 Hif1a Cell Signaling Technology Cat# 36169; RRID: AB_2799095 p-eIF2a Cell Signaling Technology Cat# 9721s; RRID: AB_330951 eIF2a Cell Signaling Technology Cat# 9722s; RRID: AB_2230924 p-GCN2 Abcam Cat# ab75836; RRID: AB_1310260 Chemicals, peptides, and recombinant proteins Recombinant Murine M-CSF Pepro Tech Cat# 315-02 Thioglycollate BD BBL Cat# 211716 Recombinant murine IL4 R&D Systems Cat# 404-ML Trizol Thermo Fisher Cat# 15596018 Recombinant murine IL10 R&D Systems Cat# 417-ML Recombinant murine IFN-g R&D Systems Cat# 485-ML Lipopolysaccharide Sigma Cat# L2630 SYBR Green Mixture TaKaRa Cat# RR420A AMV Reverse transcriptase TaKaRa Cat# 2621 Oligo dT18 Thermo Fisher Cat# 18418020 Recombinant RNase Inhibitor TaKaRa Cat# 2313A (Continued on next page) 16 Cell Reports 43, 113658, January 23, 2024

Techniques: Flow Cytometry, Expressing, Real-time Polymerase Chain Reaction, Marker

Figure 6. GCN2 is involved in T1D mouse delayed skin wound healing (A) Wound-healing pictures from control, T1D WT, and GCN2KO mice. (B) Wound-healing rates in the control group, WT T1D group, and GCN2KO group (n = 5). (C) Skin tissue H&E staining from the control group, WT T1D group, and GCN2KO group at 7 dpi (n = 3). Scale bar represents 100 mm. (D and E) Immunohistochemical staining of CD31 (D) and a-SMA (E) in skin wounds from the control group, WT T1D group, and GCN2KO group at 7 dpi (n = 3). Scale bars represent 500 mm (top) and 50 mm (bottom). (F) Flow cytometry analysis of CD206 expression in 3 or 7 dpi skin macrophages from the control group, WT T1D group, and GCN2KO group (n = 3). (G) Real-time PCR showing CD206, Il10, Tgfb1, and Vegfa expression levels in 3 or 7 dpi skin macrophages from the control group, WT T1D group, and GCN2KO group (n = 3). Data represent three independent experiments and are presented as mean ± SD. Each independent experiment consists of at least three technical replicates. *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA. See also Figure S6.

Journal: Cell reports

Article Title: Downregulation of nutrition sensor GCN2 in macrophages contributes to poor wound healing in diabetes.

doi: 10.1016/j.celrep.2023.113658

Figure Lengend Snippet: Figure 6. GCN2 is involved in T1D mouse delayed skin wound healing (A) Wound-healing pictures from control, T1D WT, and GCN2KO mice. (B) Wound-healing rates in the control group, WT T1D group, and GCN2KO group (n = 5). (C) Skin tissue H&E staining from the control group, WT T1D group, and GCN2KO group at 7 dpi (n = 3). Scale bar represents 100 mm. (D and E) Immunohistochemical staining of CD31 (D) and a-SMA (E) in skin wounds from the control group, WT T1D group, and GCN2KO group at 7 dpi (n = 3). Scale bars represent 500 mm (top) and 50 mm (bottom). (F) Flow cytometry analysis of CD206 expression in 3 or 7 dpi skin macrophages from the control group, WT T1D group, and GCN2KO group (n = 3). (G) Real-time PCR showing CD206, Il10, Tgfb1, and Vegfa expression levels in 3 or 7 dpi skin macrophages from the control group, WT T1D group, and GCN2KO group (n = 3). Data represent three independent experiments and are presented as mean ± SD. Each independent experiment consists of at least three technical replicates. *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA. See also Figure S6.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC anti-mouse CD206 BioLegend Cat# 141703; RRID: AB_10900988 PE anti-mouse F4/80 BioLegend Cat# 123110; RRID: AB_893486 APC anti-mouse F4/80 Thermo Fisher Cat# 17-4801-82; RRID: AB_2784648 PE/Cy5 anti-mouse CD11b Thermo Fisher Cat# 15-0112-83; RRID: AB_468714 Bv510 anti-mouse CD11b BioLegend Cat# 101263; RRID: AB_2629529 PE anti-mouse CD124 BioLegend Cat# 144804; RRID: AB_2561730 FITC anti-mouse F4/80 Thermo Fisher Cat# 11-4801-82; RRID: AB_2637191 PE anti-mouse TNFa Thermo Fisher Cat# 12-7321-82; RRID: AB_466199 FITC anti-mouse CD86 eBioscience Cat# 105006; RRID: AB_313149 PE/Cy5 anti-mouse CD45 Thermo Fisher Cat# 15-0459-42; RRID: AB_10717815 APC anti-mouse CD45 BioLegend Cat# 103112; RRID: AB_312977 PE anti-mouse Ly6G BioLegend Cat# 127607; RRID: AB_1186104 PE anti-mouse CD115 BioLegend Cat# 135506; RRID: AB_1937253 PE/Cy7 anti-mouse CD45.1 BioLegend Cat# 110730; RRID: AB_1134168 Fixable Viability Dye eFluorTM 780 Thermo Fisher Cat# 65-0865-14; RRID: —— PERCP/CY5.5 anti-mouse CD45.2 BioLegend Cat# 109827; RRID: AB_893352 Brilliant Violet 510 anti-mouse CD11b BioLegend Cat# 101245; RRID: AB_2561390 Brilliant Violet 605 anti-mouse Ly6C BioLegend Cat# 128036; RRID: AB_2562353 b-actin Sigma Cat# A5441; RRID: AB_476744 Arginase1 Santa Cruz Cat# sc-20150; RRID: AB_2058955 GCN2 Cell Signaling Technology Cat# 3302s; RRID: AB_10694800 p-IkBa Cell Signaling Technology Cat# 92456s; RRID: AB_2267145 IkBa Abcam Cat# ab32518; RRID: AB_2801653 p-IKKa/b Cell Signaling Technology Cat# 14938; RRID: AB_2798653 IKKa Cell Signaling Technology Cat# 61294s; RRID: AB_2799606 P65 Cell Signaling Technology Cat# 6596s; RRID: AB_2128043 p-P65 Cell Signaling Technology Cat# 3033T; RRID: AB_331284 Hif1a Cell Signaling Technology Cat# 36169; RRID: AB_2799095 p-eIF2a Cell Signaling Technology Cat# 9721s; RRID: AB_330951 eIF2a Cell Signaling Technology Cat# 9722s; RRID: AB_2230924 p-GCN2 Abcam Cat# ab75836; RRID: AB_1310260 Chemicals, peptides, and recombinant proteins Recombinant Murine M-CSF Pepro Tech Cat# 315-02 Thioglycollate BD BBL Cat# 211716 Recombinant murine IL4 R&D Systems Cat# 404-ML Trizol Thermo Fisher Cat# 15596018 Recombinant murine IL10 R&D Systems Cat# 417-ML Recombinant murine IFN-g R&D Systems Cat# 485-ML Lipopolysaccharide Sigma Cat# L2630 SYBR Green Mixture TaKaRa Cat# RR420A AMV Reverse transcriptase TaKaRa Cat# 2621 Oligo dT18 Thermo Fisher Cat# 18418020 Recombinant RNase Inhibitor TaKaRa Cat# 2313A (Continued on next page) 16 Cell Reports 43, 113658, January 23, 2024

Techniques: Control, Staining, Immunohistochemical staining, Flow Cytometry, Expressing, Real-time Polymerase Chain Reaction

Figure 7. GCN2 agonist halofuginone (HF) rescued T1D mouse delayed wound healing (A) Immunoblot analysis showing GCN2, eIF2a, and their phosphorylated versions in skin wound macrophages from control and T1D mice treated with or without HF (n = 3). (B) Wound-healing pictures from control and T1D mice treated with or without HF. (C) Wound-healing rates in control and T1D mice treated with or without HF (n = 5). (D) Skin tissue H&E staining from control and T1D mice treated with or without HF at 7 dpi (n = 3). Scale bar represents 100 mm. (E and F) Immunohistochemical staining of CD31 (E) and a-SMA (F) in skin wounds from control and T1D mice treated with or without HF at 7 dpi (n = 3). Scale bars represent 500 mm (top) and 50 mm (bottom). (G) Flow cytometry analysis of CD206 expression in skin wound macrophages from control and T1D mice treated with or without HF at 7 dpi (n = 3). (H) Real-time PCR showing CD206, Il10, Tgfb1, and Vegfa expression levels in skin macrophages from control and T1D mice treated with or without HF at 3 or 7 dpi (n = 3). Data represent three independent experiments and are presented as mean ± SD. Each independent experiment consists of at least three technical replicates. **p < 0.01, ***p < 0.001, one-way ANOVA. See also Figure S7.

Journal: Cell reports

Article Title: Downregulation of nutrition sensor GCN2 in macrophages contributes to poor wound healing in diabetes.

doi: 10.1016/j.celrep.2023.113658

Figure Lengend Snippet: Figure 7. GCN2 agonist halofuginone (HF) rescued T1D mouse delayed wound healing (A) Immunoblot analysis showing GCN2, eIF2a, and their phosphorylated versions in skin wound macrophages from control and T1D mice treated with or without HF (n = 3). (B) Wound-healing pictures from control and T1D mice treated with or without HF. (C) Wound-healing rates in control and T1D mice treated with or without HF (n = 5). (D) Skin tissue H&E staining from control and T1D mice treated with or without HF at 7 dpi (n = 3). Scale bar represents 100 mm. (E and F) Immunohistochemical staining of CD31 (E) and a-SMA (F) in skin wounds from control and T1D mice treated with or without HF at 7 dpi (n = 3). Scale bars represent 500 mm (top) and 50 mm (bottom). (G) Flow cytometry analysis of CD206 expression in skin wound macrophages from control and T1D mice treated with or without HF at 7 dpi (n = 3). (H) Real-time PCR showing CD206, Il10, Tgfb1, and Vegfa expression levels in skin macrophages from control and T1D mice treated with or without HF at 3 or 7 dpi (n = 3). Data represent three independent experiments and are presented as mean ± SD. Each independent experiment consists of at least three technical replicates. **p < 0.01, ***p < 0.001, one-way ANOVA. See also Figure S7.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies FITC anti-mouse CD206 BioLegend Cat# 141703; RRID: AB_10900988 PE anti-mouse F4/80 BioLegend Cat# 123110; RRID: AB_893486 APC anti-mouse F4/80 Thermo Fisher Cat# 17-4801-82; RRID: AB_2784648 PE/Cy5 anti-mouse CD11b Thermo Fisher Cat# 15-0112-83; RRID: AB_468714 Bv510 anti-mouse CD11b BioLegend Cat# 101263; RRID: AB_2629529 PE anti-mouse CD124 BioLegend Cat# 144804; RRID: AB_2561730 FITC anti-mouse F4/80 Thermo Fisher Cat# 11-4801-82; RRID: AB_2637191 PE anti-mouse TNFa Thermo Fisher Cat# 12-7321-82; RRID: AB_466199 FITC anti-mouse CD86 eBioscience Cat# 105006; RRID: AB_313149 PE/Cy5 anti-mouse CD45 Thermo Fisher Cat# 15-0459-42; RRID: AB_10717815 APC anti-mouse CD45 BioLegend Cat# 103112; RRID: AB_312977 PE anti-mouse Ly6G BioLegend Cat# 127607; RRID: AB_1186104 PE anti-mouse CD115 BioLegend Cat# 135506; RRID: AB_1937253 PE/Cy7 anti-mouse CD45.1 BioLegend Cat# 110730; RRID: AB_1134168 Fixable Viability Dye eFluorTM 780 Thermo Fisher Cat# 65-0865-14; RRID: —— PERCP/CY5.5 anti-mouse CD45.2 BioLegend Cat# 109827; RRID: AB_893352 Brilliant Violet 510 anti-mouse CD11b BioLegend Cat# 101245; RRID: AB_2561390 Brilliant Violet 605 anti-mouse Ly6C BioLegend Cat# 128036; RRID: AB_2562353 b-actin Sigma Cat# A5441; RRID: AB_476744 Arginase1 Santa Cruz Cat# sc-20150; RRID: AB_2058955 GCN2 Cell Signaling Technology Cat# 3302s; RRID: AB_10694800 p-IkBa Cell Signaling Technology Cat# 92456s; RRID: AB_2267145 IkBa Abcam Cat# ab32518; RRID: AB_2801653 p-IKKa/b Cell Signaling Technology Cat# 14938; RRID: AB_2798653 IKKa Cell Signaling Technology Cat# 61294s; RRID: AB_2799606 P65 Cell Signaling Technology Cat# 6596s; RRID: AB_2128043 p-P65 Cell Signaling Technology Cat# 3033T; RRID: AB_331284 Hif1a Cell Signaling Technology Cat# 36169; RRID: AB_2799095 p-eIF2a Cell Signaling Technology Cat# 9721s; RRID: AB_330951 eIF2a Cell Signaling Technology Cat# 9722s; RRID: AB_2230924 p-GCN2 Abcam Cat# ab75836; RRID: AB_1310260 Chemicals, peptides, and recombinant proteins Recombinant Murine M-CSF Pepro Tech Cat# 315-02 Thioglycollate BD BBL Cat# 211716 Recombinant murine IL4 R&D Systems Cat# 404-ML Trizol Thermo Fisher Cat# 15596018 Recombinant murine IL10 R&D Systems Cat# 417-ML Recombinant murine IFN-g R&D Systems Cat# 485-ML Lipopolysaccharide Sigma Cat# L2630 SYBR Green Mixture TaKaRa Cat# RR420A AMV Reverse transcriptase TaKaRa Cat# 2621 Oligo dT18 Thermo Fisher Cat# 18418020 Recombinant RNase Inhibitor TaKaRa Cat# 2313A (Continued on next page) 16 Cell Reports 43, 113658, January 23, 2024

Techniques: Western Blot, Control, Staining, Immunohistochemical staining, Flow Cytometry, Expressing, Real-time Polymerase Chain Reaction

FIGURE 3. bmEos exhibit chemotaxis toward recombinant mouse eotaxin-1. bmEos from day 10 cultures were used to measure chemotaxis in the presence of a mouse eotaxin-1 gradient (f), determined as percent- age of cells migrating over control (no eotaxin); chemokinesis (migration measured in response to eotaxin-1 in both chambers) is indicated by . Indistinguishable results were obtained using bmEos from day 12 cultures. Mean SEM of three separate cultures.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Functionally competent eosinophils differentiated ex vivo in high purity from normal mouse bone marrow.

doi: 10.4049/jimmunol.181.6.4004

Figure Lengend Snippet: FIGURE 3. bmEos exhibit chemotaxis toward recombinant mouse eotaxin-1. bmEos from day 10 cultures were used to measure chemotaxis in the presence of a mouse eotaxin-1 gradient (f), determined as percent- age of cells migrating over control (no eotaxin); chemokinesis (migration measured in response to eotaxin-1 in both chambers) is indicated by . Indistinguishable results were obtained using bmEos from day 12 cultures. Mean SEM of three separate cultures.

Article Snippet: Recombinant mouse eotaxin (R&D Systems) was dissolved in 0.5%BSA/PBS to 10 g/ml and then further diluted in medium containing rmIL-5 until the concentrations used in the assay were achieved.

Techniques: Chemotaxis Assay, Recombinant, Control, Migration